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产品描述：
       DNA凝胶回收试剂盒(DNA Gel Extraction Kit)，是一种用于从DNA琼脂糖凝胶中回收目的DNA的试剂盒。
  本试剂盒采用了融胶液，可以使琼脂糖凝胶完全融化。同时采用了一种新型的离子交换柱，在特定条件下，使DNA能在离心过柱的瞬间，结合到DNA纯化柱上，在一定条件下又能将DNA充分洗脱，从而实现DNA的快速纯化。无需酚氯仿抽提，无需酒精沉淀，通常12个样品只需不足20分钟即可完成。
  每个DNA纯化柱可以结合的DNA量的上限约为15微克。
  适用于从DNA琼脂糖凝胶(agarose gel)电泳后割取的含有目的DNA的凝胶块中快速提取DNA。该目的DNA通常为PCR产物、质粒DNA酶切出来的DNA片段、超螺旋质粒DNA单酶切后的线性化产物和DNA连接产物等。
  本试剂盒适用于纯化100bp-10kb DNA。长至30个碱基的引物均可被完全去除。
       DNA回收效率通常为60-90%。接近100bp或10kb的DNA片段回收效率要略低一些，大于10kb的DNA回收效率迅速下降。另外如果样品中DNA含量特别低也会导致回收效率下降。
  本试剂盒纯化所得DNA可直接用于酶切，连接，转化细菌，测序，PCR，杂交等后续操作。
  每个试剂盒足够用于50个平均重量不超过400微克的凝胶样品。
保存条件：
  室温保存，一年有效。
 

DNA提取试剂盒









 

使用本产品的文献：
1. Wen-guang Zhou, Yu-tuo Wei, Kun Huang and Ri-bo Huang.
Cloning and expression of glycerol dehydratase gene from Klebsiella pneumoniae in Escherichia coli.
J IND MICROBIOL BIOT . 2004; 34(2): 1-4. (IF 2.993)
2. Wang B, Zhang J, Duan C, Wang D.
Primary Study on Sound Effecting the Gene Expressions of Arabidopsis thaliana.
重庆大学学报（自然科学版） . 2005; 8(11): 138-141.
3. Li K, Chen L, Zhou Z, Li E, Zhao X, Guo H.
The site of vitellogenin synthesis in Chinese mitten-handed crab Eriocheir sinensis.
COMP BIOCHEM PHYS B . 2006 Apr;143(4):453-8. (IF 1.99)
4. Yuan DD, Han YH, Wang CG, Chi CW.
From the identification of gene organization of alpha conotoxins to the cloning of novel toxins.
Toxicon . 2007 Jun 15;49(8):1135-49. (IF 2.276)
5. Zhou B, Huang C, Yang J, Lu J, Dong Q, Sun LZ.
Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivomismatch repair activity assay.
Anal Biochem . 2009 May 1;388(1):167-9. (IF 2.507)
6. Yongjin J. Zhou, Fan Yang, Sufang Zhang, Haidong Tan and Zongbao K. Zhao.
Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with longhomologous arms prepared by the restriction-freecloningstrategy.
WORLD J MICROB BIOT . 2011 Dec;27(12):2999-3000. (IF 2.652)
7. Zhou Y, Wang L, Yang F, Lin X, Zhang S, Zhao ZK.
Determining the extremes of the cellular NAD(H) level by using an Escherichia coliNAD(+)-auxotrophic mutant.
APPL ENVIRON MICROB . 2011 Sep;77(17):6133-40. (IF 4.077)
8. Guo N, Chen R, Li Z, Liu Y, Cheng D, Zhou Q, Zhou J, Lin Q.
Hepatitis C virus core upregulates the methylation status of the RASSF1A promoterthroughregulation of SMYD3 in hilar cholangiocarcinoma cells.
ACTA BIOCH BIOPH SIN . 2011 May;43(5):354-61. (IF 2.502)
9. Zhang CW, Zhang XL, Xia YJ, Cao YX, Wang WJ, Xu P, Che YN, Wu XK, Yi L, Gao Q, Wang Y.
Association between polymorphisms of the CYP11A1 gene and polycystic ovary syndromein Chinese women.
Mol Biol Rep . 2012 Aug;39(8):8379-85. (IF 2.107)
10. Qian J, Wu J, Yao B, Lu Y.
Preparation ofa polyclonal antibody against hypericin synthase and localization of theenzyme in red-pigmentedHypericum perforatum L. plantlets.
Acta Biochim Pol . 2012;59(4):639-45. (IF 1.626)
11. Zhou H, Chen S, Wang W, Wang Z, Wu X, Zhang Z.
Nanog inhibits lipopolysaccharide-induced expression of pro-inflammatory cytokines byblocking NF-κBtranscriptional activity in rat primary microglial cells.
Mol Med Rep . 2012 Mar;5(3):842-6. (IF 1.851)
12. Hu D, Ju X, Li L, Hu C, Yan L, Wu T, Fu J, Qin M.
Improved in situ saccharification of cellulose pretreated by dimethyl sulfoxide/ionic liquid using cellulase from a newly isolated Paenibacillus sp. LLZ1.
BIORESOURCE TECHNOL . 2015 Nov 24;201:8-14. (IF 6.669)
13. Fu S, Fan L, Pan X, Sun Y, Zhao H.
Integrin αv promotes proliferation by activating ERK 1/2 in the human lung cancer cell line A549.
Mol Med Rep . 2015 Feb;11(2):1266-71. (IF 1.851)
14. Hu D, Ju X, Li L, Hu C, Yan L, Wu T, Fu J, Qin M.
Improved in situ saccharification of cellulose pretreated by dimethyl sulfoxide/ionic liquid using cellulasefrom a newly isolated Paenibacillus sp. LLZ1.
BIORESOURCE TECHNOL . 2016 Feb;201:8-14. (IF 6.669)
15. He WT, Zhang LM, Li C, Li SY, Ding ZC, Fang ZM, Meng FY, Chen ZK, Zhou P.
Short-term MyD88 inhibition ameliorates cardiac graft rejection and promotes donor-specifichyporesponsiveness of skin grafts in mice.
Transpl Int . 2016 Aug;29(8):941-52. (IF 3.526)
16. Zhou ZJ, Sun L.
Edwardsiella tarda-Induced Inhibition of Apoptosis: A Strategy for Intracellular Survival.
FRONT CELL INFECT MI . 2016 Jul 14;6:76. (IF 3.518)
17. Liu X, Nie S, Yu Q, Wang X, Huang D, Xie M.
Downregulation of steroid hormone receptor expression and activation of cell signal transduction pathwaysinduced by a chiral nonylphenol isomer in mouse sertoli TM4 cells.
Environ Toxicol . 2017 Feb;32(2):469-476. (IF 2.649)
18. Du X,Shen T,Wang H,Qin X,Xing D,Ye Q,Shi Z,Fang Z,Zhu Y,Yang Y,Peng Z,Zhao C,Lv B,Li X,Liu G,Li X
Adaptations of hepatic lipid metabolism and mitochondria in dairy cows with mild fatty liver.
J Dairy Sci . 2018 Oct;101(10):9544-9558. (IF 3.082)
19. Jin G,Chen Z,Zhang J,Song J,Shi J,Zhou B
Association of brain natriuretic peptide gene polymorphisms with chronic obstructive pulmonary disease complicated with pulmonary hypertension and its mechanism.
BIOSCIENCE REP . 2018 Oct 2;38(5). pii: BSR20180905. (IF 2.535)